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empty pgl3-basic  (Sangon Biotech)

 
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    Sangon Biotech empty pgl3-basic
    Empty Pgl3 Basic, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/empty pgl3-basic/product/Sangon Biotech
    Average 90 stars, based on 1 article reviews
    empty pgl3-basic - by Bioz Stars, 2026-03
    90/100 stars

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    (a) Schematic diagram for constructing wild-type or mutant promoter at the ABCG1 rs57137919G>A polymorphism site. Two constructs were subcloned into <t>pGL3-basic</t> plasmid vectors with firefly luciferase reporter gene. pG, -367G (open bar); pA, -367A (grey bar). Luciferase activity assays for two ABCG1 promoter constructs in HEK293T cells (b), THP-1 cells (c), and HepG2 cells (d) are shown. Luciferase activities were measured with the dual-luciferase reporter assay system and normalized to Renilla luciferase activity 24 hours after transfection in the presence or absence of TO901317. The luciferase activity was a corrected relative value. Data are shown as mean ± SD of four independent experiments in triplicate. P <0.01 vs. pG in HEK293 cells, THP-1 cells, and HepG2 cells under both basal state and LXR agonist stimulation.
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    the empty pgl3 basic vector was used as the negative control  (Promega)
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    Promega the empty pgl3 basic vector was used as the negative control
    Dual-luciferase reporter assay in the 293T cell line. The <t>pGL3-Basic</t> and pGL3-Promoter vectors were used as negative and positive controls, respectively. The relative luciferase level is displayed for pGL3-Promoter, pGL3-Basic and pGL3-EED The pGL3-EED indicates the recombinant EED fragment ligated to the pGL3-Basic vector. An analysis of variance and Bonferroni's correction were applied for statistical analysis. P<0.05 was considered to indicate a statistically significant difference. *P<0.0001. EED, embryonic ectoderm development; Luc, luciferase.
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    Image Search Results


    (a) Schematic diagram for constructing wild-type or mutant promoter at the ABCG1 rs57137919G>A polymorphism site. Two constructs were subcloned into pGL3-basic plasmid vectors with firefly luciferase reporter gene. pG, -367G (open bar); pA, -367A (grey bar). Luciferase activity assays for two ABCG1 promoter constructs in HEK293T cells (b), THP-1 cells (c), and HepG2 cells (d) are shown. Luciferase activities were measured with the dual-luciferase reporter assay system and normalized to Renilla luciferase activity 24 hours after transfection in the presence or absence of TO901317. The luciferase activity was a corrected relative value. Data are shown as mean ± SD of four independent experiments in triplicate. P <0.01 vs. pG in HEK293 cells, THP-1 cells, and HepG2 cells under both basal state and LXR agonist stimulation.

    Journal: PLoS ONE

    Article Title: ABCG1 rs57137919G>A Polymorphism Is Functionally Associated with Varying Gene Expression and Apoptosis of Macrophages

    doi: 10.1371/journal.pone.0097044

    Figure Lengend Snippet: (a) Schematic diagram for constructing wild-type or mutant promoter at the ABCG1 rs57137919G>A polymorphism site. Two constructs were subcloned into pGL3-basic plasmid vectors with firefly luciferase reporter gene. pG, -367G (open bar); pA, -367A (grey bar). Luciferase activity assays for two ABCG1 promoter constructs in HEK293T cells (b), THP-1 cells (c), and HepG2 cells (d) are shown. Luciferase activities were measured with the dual-luciferase reporter assay system and normalized to Renilla luciferase activity 24 hours after transfection in the presence or absence of TO901317. The luciferase activity was a corrected relative value. Data are shown as mean ± SD of four independent experiments in triplicate. P <0.01 vs. pG in HEK293 cells, THP-1 cells, and HepG2 cells under both basal state and LXR agonist stimulation.

    Article Snippet: The pGL3-basic empty vector was used as a negative control and the pGL3-control vector (Promega) was used as a positive control for the luciferase assay.

    Techniques: Mutagenesis, Construct, Plasmid Preparation, Luciferase, Activity Assay, Reporter Assay, Transfection

    Dual-luciferase reporter assay in the 293T cell line. The pGL3-Basic and pGL3-Promoter vectors were used as negative and positive controls, respectively. The relative luciferase level is displayed for pGL3-Promoter, pGL3-Basic and pGL3-EED The pGL3-EED indicates the recombinant EED fragment ligated to the pGL3-Basic vector. An analysis of variance and Bonferroni's correction were applied for statistical analysis. P<0.05 was considered to indicate a statistically significant difference. *P<0.0001. EED, embryonic ectoderm development; Luc, luciferase.

    Journal: Oncology Letters

    Article Title: Significant association of EED promoter hypomethylation with colorectal cancer

    doi: 10.3892/ol.2019.10432

    Figure Lengend Snippet: Dual-luciferase reporter assay in the 293T cell line. The pGL3-Basic and pGL3-Promoter vectors were used as negative and positive controls, respectively. The relative luciferase level is displayed for pGL3-Promoter, pGL3-Basic and pGL3-EED The pGL3-EED indicates the recombinant EED fragment ligated to the pGL3-Basic vector. An analysis of variance and Bonferroni's correction were applied for statistical analysis. P<0.05 was considered to indicate a statistically significant difference. *P<0.0001. EED, embryonic ectoderm development; Luc, luciferase.

    Article Snippet: The empty pGL3 basic vector was used as the negative control and the pGL3 promoter vector (both Promega Cooperation, Madison, WI, USA) was used as the positive control, which contained an SV40 promoter upstream of the luciferase gene.

    Techniques: Luciferase, Reporter Assay, Recombinant, Plasmid Preparation